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Trace elements solution

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Internal Solution-ID: S1782

Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(Aldrich N8407) 		9.550 g 9.55 49.963
MgCl2 x 6 H2O 4.060 g 4.06 19.97
FeCl2 x 4 H2O 0.980 g 0.98 4.929
Na2WO4 x 6 H2O 0.264 g 0.264 -
NiCl2 x 6 H2O 0.118 g 0.118 0.911
Na2Se2O3 0.100 g 0.1 -
Na2MoO4 x 2 H2O 0.024 g 0.024 0.099
CoCl2 x 6 H2O 0.023 g 0.023 0.177
Water
(Distilled or deionized) 		1000.000 ml - -
1 Adjust pH of nitrilotriacetic acid in solution to 7.0 with KOH. Add minerals and adjust the final pH to 7.0.
863 METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM