Equipment needed: Serum bottle
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Basal salts solution | 100 | ml | - | - | |
| Trace elements solution | 10 | ml | - | - | |
| Sodium carbonate solution | 24 | ml | 59.764 | - | |
| Cysteine solutions | 5 | ml | - | - | |
| Na2S Solution | 5 | ml | - | - | |
|
Distilled water
(degassed) |
856 | ml | - | - | |
| 1 Anaerobically dispense liquid minimal medium in 10-ml portions into 100-ml serum vials under an atmosphere of N2/CO2 (80:20). Seal vials with butyl rubber stoppers secured with aluminium crimp seals. Sterilize by autoclaving for 15 minutes at 121°C. Prior to inoculation, pressurize bottles to 2 x 102 kPa with H2/CO2. For solidified plating medium, add the following components per liter of liquid minimal medium: | |||||
| Agar | 15 | g | 14.764 | 43.897 | |
| Titanium (III) citrate solution (Zehnder and Wuhrmann 1976) | 16 | ml | - | - | |
| 2 Add titanium(III) citrate solution to warm (60°C) agar medium after autoclaving. For preparation of solidified agar medium. | |||||
Media with this solution
| 863 | METHANOTHERMOBACTER/METHANOBACTERIUM TRANSDUCTION MEDIUM |