Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD BBL) |
5.0 | g | 5 | - | |
|
Meat peptone
(pepsin-digested) |
5.0 | g | 5 | - | |
| Yeast extract | 10.0 | g | 10 | - | |
| NaCl | 8.0 | g | 8 | 136.893 | |
| Salt solution | 40.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| D-Glucose | 1.0 | g | 1 | 5.551 | |
| Na2S2O3 x 5 H2O | 2.5 | g | 2.5 | 10.073 | |
| Distilled water | 960.0 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine, glucose and thiosulfate), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose and thiosulfate from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 7.0, if necessary. | |||||
Media with this solution
| 104a | ANAEROBACULUM MEDIUM |