Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.2 | g | 0.2 | 1.467 | |
| MgCl2 x 6 H2O | 0.1 | g | 0.1 | 0.491 | |
| NH4Cl | 1.0 | g | 0.998 | 18.658 | |
| KCl | 0.2 | g | 0.2 | 2.677 | |
| Na2SO4 | 5.0 | g | 4.99 | 35.131 | |
| NaCl | 10.0 | g | 9.98 | 170.774 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Yeast extract | 1.0 | g | 0.998 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 3.5 | g | 3.493 | 32.957 | |
| Na2S x 9 H2O | 0.5 | g | 0.499 | 2.078 | |
| Na-formate | 4.0 | g | 3.992 | 58.7 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve the ingredients (except carbonate, sulfide, formate and vitamins) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add and dissolve carbonate and sulfide, then adjust pH to 8.8 - 9.0, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use add formate and vitamins (sterilized by filtration) from sterile anoxic stock solutions prepared under 100% N2 gas. | |||||
Media with this solution
| 813 | DESULFONATRONUM MEDIUM |