Main sol. 806
Internal Solution-ID: S1637
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KCl | 0.33 | g | 0.33 | 4.427 | |
| MgCl2 x 2 H2O | 2.70 | g | 2.7 | 20.573 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.4 | 13.794 | |
| NH4Cl | 0.25 | g | 0.25 | 4.674 | |
| CaCl2 x 2 H2O | 0.14 | g | 0.14 | 0.952 | |
| K2HPO4 | 0.14 | g | 0.14 | 0.804 | |
| Na2SeO3 | 1.00 | µg | 1.0e-6 | 5.8e-6 | |
| NiCl2 x 6 H2O | 1.00 | µg | 1.0e-6 | 7.7e-6 | |
| NaHCO3 | 1.00 | g | 1 | 11.904 | |
| NaCl | 18.00 | g | 18 | 308.008 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Yeast extract | 1.00 | g | 1 | - | |
| Trypticase | 1.00 | g | 1 | - | |
| Sulphur | 5.00 | g | 5 | - | |
| Modified Wolin's mineral solution II | 10.00 | ml | - | - | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Distilled water | 980.00 | ml | - | - | |
| 1 Prepare the medium without NaHCO3, cysteine hydrochloride, Na2S x 9 H2O, and vitamin solution. Boil the medium and cool under N2. Add the NaHCO3 to the cooled medium and adjust the pH to about pH 7.2. Sterilise the medium at 100°C for 3 hours on 3 consecutive days. Reduce the medium by adding 10% neutralised sodium sulphide and cysteine hydrochloride from sterile stock solutions. The vitamins are added from a sterile (filter sterilised) stock solution prepared under nitrogen. The final pH is 7.2. | |||||
Media with this solution
| 806 | THERMOCOCCUS MEDIUM |