Main sol. 88a
Internal Solution-ID: S156
Equipment needed: Autoclave
Water bath
Gassing station
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
| FeCl3 x 6 H2O | 0.02 | g | 0.02 | 0.074 | |
| Allen's trace element solution | 10.00 | ml | - | - | |
|
Sulfur
(powder) |
10.00 | g | 10 | 311.818 | |
|
Yeast extract
(OXOID) |
0.50 | g | 0.5 | - | |
| Na2S x 9 H2O | 0.50 | g | 0.5 | 2.082 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except sulfur, yeast extract and sulfide), adjust pH of the salt solution at room temperature to 4.0 using 1 N H2SO4 and sparge medium with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials that contain already the appropriate amount of sulfur. Sterilize medium by heating cultivation vessels in a boiling water bath for 1 - 2 hours on each of 3 successive days. Add yeast extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to inoculation check pH and adjust to 4.0, if necessary. | |||||
Media with this solution
| 88a | SULFOLOBUS MEDIUM (ANAEROBIC) |