Main sol. 641

Equipment needed: Filter Autoclave Hungate tubes Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NH₄Cl	1.0	g	0.997	18.639
Na₂SO₄	2.0	g	1.994	14.038
Na₂S₂O₃ x 5 H₂O	1.0	g	0.997	4.017
MgSO₄ x 7 H₂O	1.0	g	0.997	4.045
CaCl₂ x 2 H₂O	0.1	g	0.1	0.678
KH₂PO₄	0.5	g	0.499	3.663
Trace element solution SL-10	1.0	ml	-	-
Selenite-tungstate solution	1.0	ml	-	-
Yeast extract	1.0	g	0.997	-
Sodium resazurin (0.1% w/v)	0.5	ml	5.0e-4	0.002
Na₂CO₃	1.0	g	0.997	9.407
Na-DL-lactate	2.5	g	2.493	22.243
Wolin's vitamin solution (10x)	1.0	ml	-	-
Na₂S x 9 H₂O	0.1	g	0.1	0.415
Distilled water	1000.0	ml	-	-
1 Dissolve ingredients (except carbonate, vitamins, lactate and sulfide), sparge medium with 100% N₂ gas for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving complete the medium by adding vitamins (sterilized by filtration), lactate and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas and carbonate from a sterile anoxic stock solution prepared under 80% N₂ and 20% CO₂ gas atmosphere. Adjust pH of the complete medium to 7.0 - 7.2, if necessary.
2 Note: Prior to inoculation 10-20 mg/l sodium dithionite (added from a 5% w/v solution freshly prepared under N₂ and filter-sterilized) can be added to the medium to stimulate growth at the beginning.

641	DESULFOVIBRIO (MV) MEDIUM