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Main sol. 593

Internal Solution-ID: S1273

Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 81.000 g 81 1386.04
MgCl2 7.000 g 7 73.522
MgSO4 9.600 g 9.6 79.754
CaCl2 0.360 g 0.36 3.244
KCl 2.000 g 2 26.828
NaHCO3 0.060 g 0.06 0.714
NaBr 0.026 g 0.026 0.253
Proteose peptone no. 3
(Difco)
5.000 g 5 -
Yeast extract
(Difco)
10.000 g 10 -
Glucose 1.000 g 1 5.551
Distilled water 1000.000 ml - -
Agar , for solid medium 15.000 g/l 15 44.599
1 Final pH 7.5
2 NaHCO3 is added from a sterilized stock solution when the medium has cooled. Agar is added at a concentration of 15.0 g/l for solid media.
593 10% MH MEDIUM