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Artificial sea water, 3 times concentrated

Internal Solution-ID: S1267

This solution is used in 2 media and was first defined in medium 590.

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 70.43 g 70.43 1205.17
Na2SO4 11.75 g 11.75 82.723
NaHCO3 2.88 g 2.88 34.283
KCl 1.99 g 1.99 26.694
KBr 0.29 g 0.29 2.437
MgCl2 x 6 H2O 31.86 g 31.86 156.712
CaCl2 x 2 H2O 4.35 g 4.35 29.589
H3BO3 0.08 g 0.08 1.294
Distilled water 1000.00 ml - -
1 Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled.
590 DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM
1137 RUBRITALEA MEDIUM