| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(Difco, Bacto ) |
1.0 | g | 1 | - | |
| Na2-DL-malate | 1.0 | g | 1 | 5.616 | |
| Hutner's salts | 20.0 | ml | - | - | |
| Artificial sea water, 3 times concentrated | 980.0 | ml | - | - | |
| Agar , for solid medium | 18.0 | g/l | 18 | 53.519 | |
|
NaHCO3
(8 %) |
28.4 | ml | 2.272 | 27.045 | |
| 1 pH 7.0-7.2 | |||||
| 2 Agar at a concentration of 18 g/l is added for solid media. | |||||
| 3 Prepare an 8% solution of NaHCO3 in half full 100ml serum bottles closed with a rubber septum and autoclave. Add 28.4 ml of the sterile 8% solution of NaHCO3 (2.822g/l) per litre of the sterile, cooled medium. | |||||
Media with this solution
| 590 | DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM |