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DSMZ

Strain modifications for Medium 825

1 distinct modifications were found.

Supplement medium with 0.5 g/l DL-dithiothreitol (DTT), 20 ml/l methanol (50% v/v), and 0.5 g/l 2-mercaptoethanesulfonate (coenzyme M) added to the autoclaved medium from sterile anoxic stock solutions. The stock solution of DTT has to be sterilized by filtration. Omit Na2S x 9 H2O and L-cysteine HCl x H2O. Adjust the pH of the final medium to 6.5.

Modified strains

Methanobacterium lacus DSM 24406

Annotated modifications

Na2S x 9 H2O
L-Cysteine HCl x H2O
0.5 g DL-Dithiothreitol
0.5 g 2-Mercaptoethanesulfonate
6.5
20 ml Methanol (50% v/v)
Modifications
Supplement medium with 0.5 g/l DL-dithiothreitol (DTT), 20 ml/l methanol (50% v/v), and 0.5 g/l 2-mercaptoethanesulfonate (coenzyme M) added to the autoclaved medium from sterile anoxic stock solutions. The stock solution of DTT has to be sterilized by filtration. Omit Na2S x 9 H2O and L-cysteine HCl x H2O. Adjust the pH of the final medium to 6.5.