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DSMZ

Strain modifications for Medium 756d

1 distinct modifications were found.

Use 0.02 g/l NaNO2. Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001).

Modified strains

Nitrospira moscoviensis DSM 118083
Nitrospira defluvii DSM 118082

Annotated modifications

NaNO2
0.02 g/l NaNO2
Modifications
Use 0.02 g/l NaNO2. Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001).