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Strain modifications for Medium 71

3 distinct modifications were found.

Omit thiosulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium.

Modified strains

Acidithiobacillus thiooxidans DSM 103717

Annotated modifications

Na2S2O3 x 5 H2O
10.00 g Elemental sulfur

Replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration.

Modified strains

Acidithiobacillus ferrooxidans DSM 585

Annotated modifications

Na2S2O3 x 5 H2O
5.00 g K2S4O6

Supplement medium with 10.00 mg/l FeSO4 x 7 H2O. The final pH of the medium should be 4.4.

Modified strains

Acidithiobacillus albertensis DSM 14366

Annotated modifications

4.4
10.00 mg FeSO4 x 7 H2O
Modifications
Omit thiosulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100°C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration. Supplement medium with 10.00 mg/l FeSO4 x 7 H2O. The final pH of the medium should be 4.4.