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DSMZ

Strain modifications for Medium 339a

8 distinct modifications were found.

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT).

Modified strains

Adjust pH of medium after autoclaving to 7.4 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere.

Modified strains

Caloramator uzoniensis DSM 14924
Christensenella minuta DSM 22607

Annotated modifications

7.4

Adjust pH of medium to 6.0.

Modified strains

Clostridium ragsdalei DSM 15248

Annotated modifications

6.0

Omit D-glucose.

Modified strains

Subdoligranulum variabile DSM 15176
Mahella australiensis DSM 15567

Annotated modifications

D-Glucose

Replace D-glucose with 3.2 g/l Na2-fumarate prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2.

Modified strains

Lachnotalea glycerini DSM 28816

Annotated modifications

D-Glucose
7.2
3.2 g Na2-fumarate

Replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration.

Modified strains

Clostridium herbivorans DSM 14428
Clostridium sp. DSM 100335

Annotated modifications

D-Glucose
5 g Cellobiose

Supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of complete medium to 7.2.

Modified strains

Acetatifactor muris DSM 23669

Annotated modifications

7.2
0.2 g Dithiothreitol
Modifications
Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). Adjust pH of medium after autoclaving to 7.4 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of medium to 6.0. Omit D-glucose. Replace D-glucose with 3.2 g/l Na2-fumarate prepared under 100% N2 gas and sterilized by filtration. Adjust pH of complete medium to 7.2. Replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. Supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of complete medium to 7.2.