Strain modifications for Medium 298

11 distinct modifications were found.

Replace 2,3-butanediol with 1.00 g/l acetoin.

Modified strains

Syntrophotalea acetylenica DSM 3246

Annotated modifications

2,3-butanediol
1 g Acetoin

Replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000).

Modified strains

Syntrophotalea venetiana DSM 2395

Annotated modifications

2,3-butanediol
1 g Polyethylene glycol

Replace 2,3-butanediol with 2.20 g/l Na-gluconate.

Modified strains

Anaerobium acetethylicum DSM 29698

Annotated modifications

2,3-butanediol
2.2 g Na-gluconate

Replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium.

Modified strains

Pelospora glutarica DSM 6652

Annotated modifications

+ 20 ml Clarified rumen fluid
2,3-butanediol
2.6 g Glutaric acid

Supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a Wolin's vitamin solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution.

Modified strains

Geobacter sp. DSM 11264

Annotated modifications

+ 10 ml Wolin's vitamin solution
2,3-butanediol
0.2 g Yeast extract
1.24 g Ethylene glycol

Supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration.

Modified strains

unclassified bacterium DSM 8385

Annotated modifications

1 g Yeast extract
2,3-butanediol
2 g D-Galactose

Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH.

Modified strains

Syntrophus buswellii DSM 102354

Annotated modifications

0.86 g Crotonic acid
+ 1 ml Seven vitamins solution
2,3-butanediol

Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration.

Modified strains

Syntrophobacter wolinii DSM 102355

Annotated modifications

1.25 g Na pyruvate
+ 1 ml Seven vitamins solution
2,3-butanediol

Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas).

Modified strains

Acetobacterium sp. DSM 8909

Annotated modifications

1.4 g Triethanolamine hydrochloride
+ 1 ml Seven vitamins solution
2,3-butanediol

Supplement medium with 10.00 ml/l of a Wolin's vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385).

Modified strains

Geobacter sp. DSM 17541

Annotated modifications

+ 10 ml Wolin's vitamin solution
+ 1 ml Selenite-tungstate solution

Supplement medium with 10.00 ml/l of a Wolin's vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution.

Modified strains

Propionivibrio limicola DSM 6832

Annotated modifications

+ 10 ml Wolin's vitamin solution
2,3-butanediol
1 g Quinic acid
Modifications
Replace 2,3-butanediol with 1.00 g/l acetoin. Replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). Replace 2,3-butanediol with 2.20 g/l Na-gluconate. Replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. Supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a Wolin's vitamin solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. Supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. Supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). Supplement medium with 10.00 ml/l of a Wolin's vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). Supplement medium with 10.00 ml/l of a Wolin's vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution.