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DSMZ

Strain modifications for Medium 1523

6 distinct modifications were found.

Adjust pH of complete medium to 6.0.

Modified strains

Annotated modifications

6.0

Adjust pH of complete medium to 7.6.

Modified strains

Methanobacterium formicicum DSM 1535

Annotated modifications

7.6

Increase amount of Na-acetate to 4.00 g/l.

Modified strains

Methanoculleus thermophilus DSM 2640
Methanofollis tationis DSM 2702
Methanocorpusculum aggregans DSM 3823
Methanofollis liminatans DSM 4140
Methanocorpusculum bavaricum DSM 4179
Methanoculleus palmolei DSM 4273
Methanocorpusculum sinense DSM 4274
Methanofollis liminatans DSM 10196

Annotated modifications

Na-acetate
4 g Na-acetate

Supplement medium after autoclaving with 0.30 g/l DTT (DL-dithiothreitol), 10.00 ml/l methanol (50 % v/v), and 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from filter-sterilized anoxic stock solutions prepared under N2. Omit Na2S x 9 H2O and L-cysteine HCl x H2O.

Modified strains

Methanorbis furvi DSM 115764
Methanorbis rubei DSM 115765

Annotated modifications

10 ml Methanol (50 % v/v)
0.3 g DL-Dithiothreitol
0.5 g 2-Mercaptoethanesulfonic acid
L-Cysteine HCl x H2O
Na2S x 9 H2O

Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) and 0.30 g/l DTT (DL-Dithiothreitol) added from filter-sterilized anoxic stock solutions prepared under N2. Omit sulfide and cysteine from the medium.

Modified strains

Methanobrevibacter ruminantium DSM 1093
Methanobrevibacter arboriphilus DSM 1125
Methanobrevibacter gottschalkii DSM 11977
Methanobrevibacter millerae DSM 16643
Methanobrevibacter gottschalkii DSM 11978
Methanobacterium sp. DSM 862
Methanobrevibacter intestini DSM 116060

Annotated modifications

0.3 g DL-Dithiothreitol
0.5 g 2-Mercaptoethanesulfonic acid
L-Cysteine HCl x H2O
Na2S x 9 H2O

Supplement medium after autoclaving with 50 ml/l clarified rumen fluid and 0.3 g/l DTT (DL-dithiothreitol). Omit Na2S x 9 H2O.

Modified strains

Methanobrevibacter olleyae DSM 16632

Annotated modifications

+ 50 ml Clarified rumen fluid
Na2S x 9 H2O
0.3 g DL-Dithiothreitol
Modifications
Adjust pH of complete medium to 6.0. Adjust pH of complete medium to 7.6. Increase amount of Na-acetate to 4.00 g/l. Supplement medium after autoclaving with 0.30 g/l DTT (DL-dithiothreitol), 10.00 ml/l methanol (50 % v/v), and 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) added from filter-sterilized anoxic stock solutions prepared under N2. Omit Na2S x 9 H2O and L-cysteine HCl x H2O. Supplement medium after autoclaving with 0.50 g/l coenzyme M (2-mercaptoethanesulfonic acid) and 0.30 g/l DTT (DL-Dithiothreitol) added from filter-sterilized anoxic stock solutions prepared under N2. Omit sulfide and cysteine from the medium. Supplement medium after autoclaving with 50 ml/l clarified rumen fluid and 0.3 g/l DTT (DL-dithiothreitol). Omit Na2S x 9 H2O.