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DSMZ

Strain modifications for Medium 141c

8 distinct modifications were found.

Adjust pH of complete medium to 6.8.

Modified strains

Methanosarcina siciliae DSM 6564

Annotated modifications

6.8

Adjust pH to 6.6.

Modified strains

Methanosarcina siciliae DSM 11916

Annotated modifications

6.6

Omit trimethylammonium chloride from the medium.

Modified strains

Thermosipho africanus DSM 5309
Thermosipho sp. DSM 21301
Thermosipho sp. DSM 21708
Thermosipho africanus DSM 24758

Annotated modifications

Trimethylammonium chloride

Replace trimethylammonium chloride with 1.00 ml/l methanol solution (50% v/v) added from a sterile anoxic stock solution prepared under 100% N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure.

Modified strains

Methanosarcina baltica DSM 14042

Annotated modifications

Trimethylammonium chloride
0.50 ml Methanol

Replace trimethylammonium chloride with 10.00 ml/l methanol solution (50% v/v) added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of complete medium to 6.8 - 7.2.

Modified strains

Methanosarcina acetivorans DSM 2834
Methanosarcina siciliae DSM 3028
Methanolobus vulcani DSM 3029
Methanosarcina mazei DSM 3318
Methanosarcina sp. DSM 6636
Methanosarcina sp. DSM 13159
Methanolobus profundi DSM 21213
Methanolobus sp. DSM 21221
Methanolobus zinderi DSM 21339
Methanosarcina sp. DSM 15192
Methanolobus psychrotolerans DSM 104044
Methanolobus sp. DSM 100371

Annotated modifications

Trimethylammonium chloride
6.8 - 7.2
5.00 ml Methanol

Replace trimethylammonium chloride with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under 100% N2 gas. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture.

Modified strains

Thermotoga sp. DSM 4138

Annotated modifications

Trimethylammonium chloride
5.00 g D-Glucose

Replace trimethylammonium chloride with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under 100% N2 gas.

Modified strains

Anaeroflexus maritimus DSM 2831

Annotated modifications

Trimethylammonium chloride
5.00 g Sucrose

Supplement the autoclaved medium with 3.00 g/l Na2S2O3 from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration.

Modified strains

Methanococcoides methylutens DSM 16625

Annotated modifications

3 g Na2S2O3
Modifications
Adjust pH of complete medium to 6.8. Adjust pH to 6.6. Omit trimethylammonium chloride from the medium. Replace trimethylammonium chloride with 1.00 ml/l methanol solution (50% v/v) added from a sterile anoxic stock solution prepared under 100% N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. Replace trimethylammonium chloride with 10.00 ml/l methanol solution (50% v/v) added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of complete medium to 6.8 - 7.2. Replace trimethylammonium chloride with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under 100% N2 gas. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. Replace trimethylammonium chloride with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under 100% N2 gas. Supplement the autoclaved medium with 3.00 g/l Na2S2O3 from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration.