PUBLIC P9
P9 .
PDB-SNG medium
User-provided medium: This medium was provided by one of our users. Please be aware that we do not curate or test the media that are composed by our users. Be cautious when trying this medium, and check the following reference carefully: DOI: 10.1007/s11104-022-05822-6
Culture medium recipe
Vol.
×
Main solution #
ml
Equipment needed: Autoclave
pH meter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 2 | g | 2 | 15.135 | |
| Distilled water | 900 | ml | - | - | |
| Fe(III) citrate | 1 | μg | - | - | |
| CuSO4 | 1 | μg | - | - | |
| Trace element solution SL-10 | 1 | ml | - | - | |
| Trace element solution SL-6 | 1 | ml | - | - | |
| Agar | 20 | g | 20 | - | |
| 1 1. Disolver todos los ingredientes del medio de cultivo en agua destilada ajustando a un litro | |||||
| 2 2. Añadir 1-2 ml de la solución de elementos traza seleccionados SL-10 o SL-6 | |||||
| 3 3. Mezclar bien hasta que de hayan distribuido uniformemente | |||||
| 4 4. Ajustar a un pH con baso o acido si es necesario | |||||
| 5 5. Esterilizar en autoclave a 121° durante 15 min | |||||
| 6 *Notas: El nivel de pH puede varía entre 6.5-8, pero si es específico (Methylococcus, Methylomanas y Methylobacter) agregando HCl o NaOH | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
| Distilled water | 1000.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Creator: | CHOUKRI Ahmed (Nantes University) |
| Author(s): | Rey Flores Ángel Geovanny, Valencia Mazón Gabriel |
| Published: | 09.01.24 |
| Last modified: | 25.07.24 |
| Source: | MediaDive: public |
| Medium type: | Complex medium |
| Final pH: | 6.8 - 8 |
| Equipment needed: | Autoclave, pH meter |
| Reference: | DOI: 10.1007/s11104-022-05822-6 |
|
Description:
Medio de cultivos para organismos quimiliototrofos de cuerpos de agua y lecho marinos con principalmente fuente de carbono el metano |
|
| Edit in Medium builder | |
Gas composition
- 10 % CH4
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .