J962
962 .
GAL1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Mobilisporobacter senegalensis DSM 26537
This strain uses the standard medium without modifications.
Main sol. J962 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.30 | g | 0.29 | 2.132 | |
| NH4Cl | 0.30 | g | 0.29 | 5.424 | |
| NaCl | 1.00 | g | 0.967 | 16.549 | |
| KCl | 0.50 | g | 0.484 | 6.486 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.387 | 1.903 | |
| CaCl2 x 2 H2O | 0.15 | g | 0.145 | 0.987 | |
| Trace element solution | 1.00 | ml | - | - | |
| Yeast extract | 0.50 | g | 0.484 | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.484 | - | |
| Resazurin | 1.00 | mg | 9.7e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly, adjust pH to 7.0 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solution from an anaerobic stock [filter-sterilized and stored under N2-CO2 (4:1, v/v)]: | |||||
|
NaHCO3
(8%) |
25.00 | ml | - | - | |
| 2 Aseptically distribute the medium into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclave and store under a N2 atmosphere): | |||||
|
Na2S x 9 H2O
(5%) |
8.00 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Mobilisporobacter senegalensis DSM 26537
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 0.967 | 16.549 |
| KCl | 0.484 | 6.486 |
| Yeast extract | 0.484 | - |
| L-Cysteine HCl x H2O | 0.484 | - |
| MgCl2 x 6 H2O | 0.387 | 1.903 |
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