Name: SULFURIMONAS HONGKONGENSIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Main sol. J941 #

Solution A #

932

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
KH₂PO₄	2	g	2.146	15.769
KNO₃	2	g	2.146	21.225
NH₄Cl	1	g	1.073	20.059
NaCl	25	g	26.824	459.001
Trace element solution SL-4	2	ml	-	-
Distilled water	930	ml	-	-
1 Adjust pH to 7.0 with NaOH.

Solution B #

10

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
MgCl₂ x 6 H₂O	0.8	g	80	393.501
Distilled water	10.0	ml	-	-

Solution C #

40

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Na₂S₂O₄ x 2 H₂O	5	g	125	-
Distilled water	40	ml	-	-

Solution D #

20

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaHCO₃	1	g	50	595.188
Distilled water	20	ml	-	-

Solution E #

1

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
FeSO₄ x 7 H₂O	0.2	mg	0.2	0.719
H₂SO₄ (0.1 N)	1.0	ml	-	-
1 Separately autoclave Solutions A, B, C and E under a N₂ atmosphere. Filter-sterilize Solution D. After sterilization, combine all solutions and store under a N₂ atmosphere.

Trace element solution SL-4 #

1000

Solution occurs in 21 other media (first defined in Medium J340).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
EDTA	0.5	g	0.5	1.711
FeSO₄ x 7 H₂O	0.2	g	0.2	0.719
Trace element solution SL-6	100.0	ml	-	-
Distilled water	900.0	ml	-	-

Trace element solution SL-6 #

1000

Solution occurs in 55 other media (first defined in Medium 27).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-

Last modified:	22.02.22
Source:	JCM
Taxonomic range:	Bacteria
Medium type:	Defined medium
Final pH:	7.0
Equipment needed:	Autoclave, Filter

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phyla	1	1 strain
oxygen	anaerobe	1 strain
oxygen	obligate aerobe	1 strain
temperature	mesophilic	1 strain

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J941
941 . SULFURIMONAS HONGKONGENSIS MEDIUM

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