J918
918 .
DESULFAMPLUS MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Desulfamplus magnetovallimortis DSM 103535
This strain uses the standard medium without modifications.
Main sol. J918 #
ml
Equipment needed: Anaerobic workbench
Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 20.00 | g | 19.455 | 332.91 | |
| MgCl2 x 6 H2O | 6.00 | g | 5.837 | 28.709 | |
| Na2SO4 | 3.24 | g | 3.152 | 22.189 | |
| CaCl2 x 2 H2O | 1.00 | g | 0.973 | 6.617 | |
| KCl | 0.55 | g | 0.535 | 7.177 | |
| NH4Cl | 0.30 | g | 0.292 | 5.456 | |
| Modified Wolfe's mineral solution | 5.00 | ml | - | - | |
| Sodium fumarate | 1.00 | g | 0.973 | 6.078 | |
| HEPES | 2.40 | g | 2.335 | 9.797 | |
| Yeast extract | 0.20 | g | 0.195 | - | |
| Resazurin | 0.40 | mg | 3.9e-4 | 0.002 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels under a stream of a N2 gas, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from anaerobic stock solutions (autoclaved or *filter-sterilized): | |||||
|
Potassium phosphate buffer
(pH 7.0, 0.5 M) |
1.80 | ml | - | - | |
|
Vitamin solution
(*) |
0.50 | ml | - | - | |
|
NaHCO3
(*, 8%) |
2.35 | ml | - | - | |
|
L-Cysteine HCl x H2O
(pH 7.0, 5%) |
8.00 | ml | - | - | |
|
FeSO4 x 5 H2O
(in 0.02 N HCl) (optional, 0.01 M) |
10.00 | ml | - | - | |
| 2 Readjust pH to 7.2 - 7.5, if necessary, with sterile HCl or NaOH solution. Inoculate when the medium turns colorless. | |||||
| 3 Comment: Optionally add the FeSO4 x 5H2O solution as described, for the formation of magnetosome. | |||||
Solution occurs in 2
other media (first defined in Medium J915).
| 1 Use trace minerals (see Medium No. 151) with final 0.4 g/L Na2MoO4 x 2H2O, 0.02 g/L CuSO4 x 5H2O and 0.01 g/L NiCl2 x 6H2O. |
Solution occurs in 2
other media (first defined in Medium J915).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Thiamine HCl | 90.0 | mg | 0.9 | - | |
| Myo-inositol | 40.0 | mg | 0.4 | 2.22 | |
| Calcium pantothenate | 4.0 | mg | 0.04 | 0.084 | |
| p-Aminobenzoic acid | 5.0 | mg | 0.05 | 0.365 | |
| Vitamin B12 | 5.0 | mg | 0.05 | 0.037 | |
| Pyridoxine hydrochloride | 4.0 | mg | 0.04 | - | |
| Nicotinic acid | 4.0 | mg | 0.04 | 0.325 | |
| Biotin | 0.1 | mg | 1.0e-3 | 0.004 | |
| Folic acid | 2.0 | mg | 0.02 | 0.045 | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Comment: The medium tubes can be stocked under a N2 gas atmosphere. In this case, prior to use, fill the head space with air and allow to stand until the O2 gradient is formed. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.3 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Desulfamplus magnetovallimortis DSM 103535
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 19.455 | 332.91 |
| MgCl2 x 6 H2O | 5.837 | 28.709 |
| Na2SO4 | 3.152 | 22.189 |
| HEPES | 2.335 | 9.797 |
| CaCl2 x 2 H2O | 0.973 | 6.617 |
| Show more | ||
Cultivation metadata from 1 strains
| phyla | 1 | 1 strain |