J900
900 .
M13a MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J900 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.25 | g | 0.25 | - | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| Glucose | 0.25 | g | 0.25 | 1.388 | |
| Vitamin solution | 10.00 | ml | - | - | |
| Modified Hutner's basal salts | 20.00 | ml | - | - | |
|
Tris-HCl buffer
(pH 7.5, 0.1 M) |
50.00 | ml | - | - | |
|
Sea water
(artificial) |
500.00 | ml | - | - | |
| Distilled water | 420.00 | ml | - | - | |
| 1 For agar plates, increase pH of Tris-HCl buffer to 8.5. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 0.2 | mg | 2.0e-4 | 1.5e-4 | |
| Biotin | 4.0 | mg | 0.004 | 0.016 | |
| Thiamine-HCl x 2 H2O | 10.0 | mg | 0.01 | - | |
| Calcium pantothenate | 10.0 | mg | 0.01 | 0.021 | |
| Folic acid | 1.0 | mg | 1.0e-3 | 0.002 | |
| Riboflavin | 10.0 | mg | 0.01 | 0.027 | |
| Nicotinamide | 10.0 | mg | 0.01 | 0.082 | |
| p-Aminobenzoic acid | 10.0 | mg | 0.01 | 0.073 | |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Filter-sterilize. | |||||
Solution occurs in 3
other media (first defined in Medium J900).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nitrilotriacetic acid
(NTA) |
10.000 | g | 10 | 52.318 | |
| MgSO4 x 7 H2O | 29.700 | g | 29.7 | 120.499 | |
| CaCl2 x 2 H2O | 3.335 | g | 3.335 | 22.685 | |
| (NH4)6Mo7O24 x 4 H2O | 9.250 | mg | 0.009 | - | |
| FeSO4 x 7 H2O | 99.000 | mg | 0.099 | 0.356 | |
| Metals ''44'' | 50.000 | ml | - | - | |
| Distilled water | 950.000 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4. | |||||
Solution occurs in 7
other media (first defined in Medium J149).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 250.0 | mg | 0.25 | - |
| ZnSO4 x 7 H2O | 1095.0 | mg | 1.095 | 3.808 |
| FeSO4 x 7 H2O | 500.0 | mg | 0.5 | 1.798 |
| MnSO4 x n H2O | 154.0 | mg | 0.154 | - |
| CuSO4 x 5 H2O | 39.2 | mg | 0.039 | 0.157 |
| Co(NO3)2 x 6 H2O | 24.8 | mg | 0.025 | 0.085 |
| Na2B4O7 x 10 H2O | 17.7 | mg | 0.018 | - |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 20 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MgSO4 x 7 H2O | 0.594 | 2.41 |
| Glucose | 0.25 | 1.388 |
| Peptone | 0.25 | - |
| Yeast extract | 0.25 | - |
| Nitrilotriacetic acid | 0.2 | 1.046 |
| Show more | ||
Cultivation metadata from 20 strains
| pH | 6 - 8 | 1 strain |
| phyla | 1 | 20 strains |
| oxygen | aerobe | 1 strain |
| temperature | mesophilic | 19 strains |