J865
865 .
DESULFOSPOROSINUS BSREI1 MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Desulfosporosinus burensis DSM 24089
This strain uses the standard medium without modifications.
Main sol. J865 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.450 | g | 0.446 | 3.375 | |
| KH2PO4 | 0.050 | g | 0.05 | 0.364 | |
| MgSO4 x 7 H2O | 0.500 | g | 0.496 | 2.011 | |
| KCl | 0.050 | g | 0.05 | 0.665 | |
| Ca(NO3)2 x 4 H2O | 0.014 | g | 0.014 | 0.059 | |
| Na2SO4 | 3.000 | g | 2.973 | 20.932 | |
| Trace element solution | 1.000 | ml | - | - | |
| Yeast extract | 0.500 | g | 0.496 | - | |
| L-Cysteine HCl x H2O | 0.500 | g | 0.496 | - | |
| Resazurin | 1.000 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 910.000 | ml | - | - | |
| 1 Mix components thoroughly, distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 910 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
NaHCO3
(*, 5%) |
60.000 | ml | - | - | |
|
Fructose
(1 M) |
20.000 | ml | - | - | |
|
Trace vitamins (see Medium No. 197)
(*) |
10.000 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
8.000 | ml | - | - | |
Solution occurs in 38
other media (first defined in Medium J439).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
HCl
(25%) |
12.5 | ml | - | - |
| FeSO4 x 7 H2O | 2.1 | g | 2.1 | 7.554 |
| H3BO3 | 30.0 | mg | 0.03 | 0.485 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | - |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 |
| ZnSO4 x 7 H2O | 144.0 | mg | 0.144 | 0.501 |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 |
| Distilled water | 987.0 | ml | - | - |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Desulfosporosinus burensis DSM 24089
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2SO4 | 2.973 | 20.932 |
| MgSO4 x 7 H2O | 0.496 | 2.011 |
| Yeast extract | 0.496 | - |
| L-Cysteine HCl x H2O | 0.496 | - |
| (NH4)2SO4 | 0.446 | 3.375 |
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