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JCM 828 . MODIFIED NMS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J828 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1.0 g 0.985 3.997
CaCl2 x 2 H2O 0.2 g 0.197 1.34
KNO3 1.0 g 0.985 9.745
Trace element solution SL-4 5.0 ml - -
Chelated iron solution 10.0 ml - -
Distilled water 900.0 ml - -
1 Mix components and autoclave. After cooling, add the following solutions (autoclaved) to the medium:
Phosphate buffer solution 100.0 ml - -
2 Adjust pH to 6.8, if necessary. Aseptically distribute the medium into sterile serum bottles (leave &; 80% gas phase by volume) and seal with rubber stoppers. Replace the gas phase with an air-methane gas mixture (75:25, v/v). Cultivate with shaking.

Chelated iron solution #

100
Solution occurs in 1 other media (first defined in Medium J828).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 0.0270 g 0.27 0.999
Na2-EDTA 0.0372 g 0.372 -
Distilled water 100.0000 ml - -

Phosphate buffer solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.272 g 2.72 19.987
Na2HPO4 x 2 H2O 0.717 g 7.17 50.508
Distilled water 100.000 ml - -

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.8
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KNO3 0.985 9.745
MgSO4 x 7 H2O 0.985 3.997
Na2HPO4 x 2 H2O 0.706 4.976
KH2PO4 0.268 1.969
CaCl2 x 2 H2O 0.197 1.34
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain