MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 778 . THERMOANAEROMONAS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Thermanaerothrix daxensis DSM 23592

This strain uses the standard medium without modifications.

Main sol. J778 #

ml
Equipment needed: Hungate tubes Filter Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.3 g 0.282 2.074
K2HPO4 0.3 g 0.282 1.62
NH4Cl 1.0 g 0.941 17.587
NaCl 1.0 g 0.941 16.097
KCl 0.1 g 0.094 1.262
CaCl2 x 2 H2O 0.1 g 0.094 0.64
MgCl2 x 6 H2O 0.5 g 0.47 2.314
Trace minerals 10.0 ml - -
Yeast extract 1.0 g 0.941 -
Trypticase peptone
(BD-BBL) 		1.0 g 0.941 -
Resazurin 1.0 mg 9.4e-4 0.004
Distilled water 1000.0 ml - -
1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
NaHCO3
(*, 8%) 		25.0 ml - -
Na2S x 9 H2O
(5.0%) 		8.0 ml - -
Glucose
(1 M) 		20.0 ml - -

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
Edit in Medium builder

Associated strains

All strains for this medium: 2
Thermanaerothrix daxensis DSM 23592

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 0.95 16.258
NH4Cl 0.941 17.587
Yeast extract 0.941 -
Trypticase peptone 0.941 -
MgCl2 x 6 H2O 0.47 2.314
Show more
CSV
JSON

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature thermophilic 2 strains