MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

JCM 760 . DESULFURISPIRILLUM ALKALIPHILUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J760 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 22.0 g 21.033 198.442
NaHCO3 8.0 g 7.648 91.042
NaCl 6.0 g 5.736 98.154
K2HPO4 0.5 g 0.478 2.744
Distilled water 1000.0 ml - -
1 Mix components and autoclave under a N2 atmosphere. Then add the following solutions from sterile anaerobic stocks:
MgSO4
(0.1 M) 		10.0 ml - -
Yeast extract
(1%) 		2.0 ml - -
Sodium acetate
(1.0 M) 		20.0 ml - -
KNO3
(1.0 M) 		10.0 ml - -
Trace element solution SL-4 1.0 ml - -
2 Mix well until clarified. Finally, add the following solution to the medium:
Na2S x 9 H2O
(5%) 		2.5 ml - -
3 Comment: Incubate with a 5-10% inoculum. The strain also grows in the medium with sulfur as an electron acceptor. In this case, use the above described medium, replacing KNO3 with 0.25 g/L NH4Cl and 2.0 g/L sulfur (separately autoclaved at 110C for 40 min).

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
Edit in Medium builder

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 21.033 198.442
NaHCO3 7.648 91.042
NaCl 5.736 98.154
K2HPO4 0.478 2.744
EDTA 4.8e-4 0.002
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain