JCM 676 . PYG MEDIUM (G)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Segatella maculosa DSM 19339

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 5.0 g 4.995 -
Peptone 5.0 g 4.995 -
Yeast extract 10.0 g 9.99 -
Beef extract 5.0 g 4.995 -
Glucose 5.0 g 4.995 27.725
K2HPO4 2.0 g 1.998 11.471
L-Cysteine HCl x H2O 0.5 g 0.5 -
Tween 80 1.0 ml - -
Resazurin 1.0 mg 1.0e-3 0.004
Salt solution 40.0 ml - -
Distilled water 940.0 ml - -
Hemin solution 10.0 ml - -
Hemin solution 10.0 ml - -
1 Adjust pH to 7.2.
Solution occurs in 9 other media (first defined in Medium 104).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.25 1.701
MgSO4 x 7 H2O 0.50 g 0.5 2.029
K2HPO4 1.00 g 1 5.741
KH2PO4 1.00 g 1 7.348
NaHCO3 10.00 g 10 119.038
NaCl 2.00 g 2 34.223
Distilled water 1000.00 ml - -
Solution occurs in 5 other media (first defined in Medium J469).
1 Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 19
Segatella maculosa DSM 19339

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 9.99 -
Glucose 4.995 27.725
Trypticase peptone 4.995 -
Peptone 4.995 -
Beef extract 4.995 -
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Cultivation metadata from 19 strains BacDive

pH 6 - 8 4 strains
pH 4 - 6 2 strains
pH 2 - 4 1 strain
pH 8 - 10 1 strain
pH 10 - 12 1 strain
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