66 .
V8 JUICE AGAR
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Selected strain: Actinoalloteichus spitiensis DSM 44848
This strain uses the standard medium without modifications.
Equipment needed:
Centrifuge
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
V8 canned vegetable juice
(Campbell) |
200 | ml | ||
|
|
CaCO3 | 3 | g | ||
|
|
Agar | 15 | g | ||
|
|
Distilled water | 800 | ml | ||
| 1 Adjust pH to 7.2. | |||||
| 2 Filter V8 vegetable juice through 4 layers of cheesecloth or centrifuge (3,000 rpm, 10 min) to eliminate fibers. | |||||