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JCM 631 . MMB MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J631 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 0.1 g 0.097 -
Yeast extract 0.1 g 0.097 -
Glucose 1.0 g 0.971 5.389
(NH4)2SO4 0.2 g 0.194 1.469
Hunter's basal salts 20.0 ml - -
Trace vitamins 10.0 ml - -
Distilled water 1000.0 ml - -
1 Adjust pH to 7.0 - 7.5.
2 For preparation of solid medium, add 15.0 g/L agar.

Hunter's basal salts #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 10.00 g 10 52.318
MgSO4 x 7 H2O 14.45 g 14.45 58.626
CaCl2 x 2 H2O 4.42 g 4.42 30.065
(NH4)6Mo7O24 x 4 H2O 9.25 mg 0.009 -
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
Nicotinic acid 50.00 mg 0.05 0.406
Thiamine HCl 25.00 mg 0.025 -
Biotin 0.50 mg 5.0e-4 0.002
Metals ''44'' 50.00 ml - -
Distilled water 950.00 ml - -
1 Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8 and bring volume to 1.0 L with distilled water.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metals ''44'' #

1000
Solution occurs in 7 other media (first defined in Medium J149).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 250.0 mg 0.25 -
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x n H2O 154.0 mg 0.154 -
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glucose 0.971 5.389
MgSO4 x 7 H2O 0.281 1.138
(NH4)2SO4 0.194 1.469
Nitrilotriacetic acid 0.194 1.016
Peptone 0.097 -
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 3 strains
oxygen aerobe 2 strains
temperature mesophilic 3 strains