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JCM 607 . HALOMONAS SUBGLACIESCOLA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J607 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 100.0 g 96.062 1643.76
MgCl2 x 6 H2O 5.0 g 4.803 23.625
MgSO4 x 7 H2O 9.5 g 9.126 37.025
KCl 5.0 g 4.803 64.428
CaCl2 x 2 H2O 0.2 g 0.192 1.307
(NH4)2SO4 0.1 g 0.096 0.727
KNO3 0.1 g 0.096 0.95
Peptone 5.0 g 4.803 -
Yeast extract 1.0 g 0.961 -
Distilled water 1000.0 ml - -
1 Adjust pH to 7.0. For preparation of solid medium, add 15.0 g/L agar. Autoclave. After cooling to 50C, add the following solutions to the medium (autoclaved or *filter-sterilized):
Metal solution 20.0 ml - -
Phosphate solution 20.0 ml - -
Trace vitamins
(*) 		1.0 ml - -

Metal solution #

1050
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 10.0 g 9.524 49.826
MgSO4 x 7 H2O 29.7 g 28.286 114.761
CaCl2 x 2 H2O 3.3 g 3.143 21.378
Na2MoO4 x 2 H2O 12.7 mg 0.012 0.05
FeSO4 x 7 H2O 99.0 mg 0.094 0.339
Metals ''44'' 50.0 ml - -
Distilled water 1000.0 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add the minerals. Adjust final pH to 7.0.

Phosphate solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 2.5 g 2.5 14.353
KH2PO4 2.5 g 2.5 18.371
Distilled water 1000.0 ml - -

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metals ''44'' #

1000
Solution occurs in 7 other media (first defined in Medium J149).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 250.0 mg 0.25 -
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x n H2O 154.0 mg 0.154 -
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.5
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 96.062 1643.76
MgSO4 x 7 H2O 9.669 39.23
KCl 4.803 64.428
MgCl2 x 6 H2O 4.803 23.625
Peptone 4.803 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain