JCM 58 . INORGANIC SALTS-STARCH AGAR (ISP-4)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Streptomyces abikoensis DSM 41491

This strain uses the standard medium without modifications.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Starch
(soluble)
10 g 9.99 -
K2HPO4 1 g 0.999 5.736
MgSO4 x 7 H2O 1 g 0.999 4.053
NaCl 1 g 0.999 17.095
(NH4)2SO4 2 g 1.998 15.12
CaCO3 2 g 1.998 19.962
Trace salts solution 1 ml - -
Agar 20 g 19.98 59.406
Distilled water 1000 ml - -
1 Unadjusted pH will be 7.0-7.4.
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 -
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -
1 Comment: The premixed powder is available from Becton Dickinson & Co. as ISP Medium 4.

Metadata

Last modified: 24.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 276
Streptomyces abikoensis DSM 41491

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 19.98 59.406
Starch 9.99 -
CaCO3 1.998 19.962
(NH4)2SO4 1.998 15.12
NaCl 0.999 17.095
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Cultivation metadata from 276 strains BacDive

pH 6 - 8 5 strains
pH 4 - 6 3 strains
pH 8 - 10 2 strains
pH 10 - 12 1 strain
phyla 2 279 strains
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