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JCM 559 . 1/2 SME MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

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Medium for Ignicoccus hospitalis DSM 18386

This strain uses the standard medium without modifications.

Main sol. J559 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 13.850 g 13.85 236.995
MgSO4 x 7 H2O 3.500 g 3.5 14.2
MgCl2 x 6 H2O 2.750 g 2.75 13.527
KH2PO4 0.500 g 0.5 3.674
(NH4)2SO4 0.250 g 0.25 1.892
KCl 0.330 g 0.33 4.427
CaCl2 x 2 H2O 0.380 g 0.38 2.585
NaBr 0.050 g 0.05 0.486
H3BO3 0.015 g 0.015 0.243
SrCl2 x 6 H2O 7.500 mg 0.008 0.028
KI 0.050 mg 5.0e-5 1.9e-4
NaHCO3 0.160 g 0.16 1.905
Sulfur
(powder) 		10.000 g 10 311.818
Resazurin 1.000 mg 1.0e-3 0.004
Na2S x 9 H2O 0.500 g 0.5 2.082
Distilled water 1000.000 ml - -
1 Mix ingredients except Na2S x 9H2O and distribute the medium into culture vessels under a stream of a N2-CO2 (4:1, v/v) gas mixture. Add Na2S x 9H2O as a 5% solution, adjust pH to 6.0 and seal with butyl rubber stoppers. Autoclave at 110C for 1 hr. Prior to cultivation, replace the gas phase with a H2-CO2 (4:1, v/v) gas mixture. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Medium type: Defined medium
Final pH: 6.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1
Ignicoccus hospitalis DSM 18386

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 13.85 236.995
Sulfur 10 311.818
MgSO4 x 7 H2O 3.5 14.2
MgCl2 x 6 H2O 2.75 13.527
KH2PO4 0.5 3.674
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Cultivation metadata from 1 strains BacDive

pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
nutrition chemolithoautotroph 1 strain
oxygen anaerobe 1 strain
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