435 .
ANAEROLINEA MEDIUM-B
What is this page?
Selected strain: Leptolinea tardivitalis DSM 16556
This strain uses the standard medium without modifications.
Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
n.d.
Select a strain (optional):
| 1 Use Solution A of Medium No. 284 supplemented with final 1.0 ml Trace vitamins solution and 1.0 g yeast extract, and dissolved in 1.0 L distilled water. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O and Na2S x 9H2O solutions to the medium (3% solutions, autoclaved and stored under a N2 atmosphere). | |||||
| 2 Comment: For strain JCM 12579, incubate with a 2% inoculum. |