J435
435 .
ANAEROLINEA MEDIUM-B
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
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Medium for Leptolinea tardivitalis DSM 16556
This strain uses the standard medium without modifications.
Equipment needed: Autoclave
Anaerobic workbench
| 1 Use Solution A of Medium No. 284 supplemented with final 1.0 ml Trace vitamins solution and 1.0 g yeast extract, and dissolved in 1.0 L distilled water. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 50 ml serum bottle) under a stream of N2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Prior to inoculation, aseptically and anaerobically add 0.01 volume of L-cysteine x HCl x H2O and Na2S x 9H2O solutions to the medium (3% solutions, autoclaved and stored under a N2 atmosphere). | |||||
| 2 Comment: For strain JCM 12579, incubate with a 2% inoculum. |
Metadata
| Last modified: | 29.10.21 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Leptolinea tardivitalis DSM 16556
No modifications necessary for this strain.
