J420
420 .
MJAIS/MD MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Thioreductor micantisoli DSM 16661
This strain uses the standard medium without modifications.
Main sol. J420 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 30.00 | g | 29.674 | 507.762 | |
| K2HPO4 | 0.09 | g | 0.089 | 0.511 | |
| KH2PO4 | 0.07 | g | 0.069 | 0.509 | |
| CaCl2 x 2 H2O | 0.80 | g | 0.791 | 5.382 | |
| NH4Cl | 1.25 | g | 1.236 | 23.115 | |
| MgSO4 x 7 H2O | 3.40 | g | 3.363 | 13.644 | |
| MgCl2 x 6 H2O | 4.18 | g | 4.135 | 20.337 | |
| KCl | 0.33 | g | 0.326 | 4.378 | |
| NiCl2 x 6 H2O | 10.00 | mg | 0.01 | 0.076 | |
| Na2SeO3 x 5 H2O | 10.00 | mg | 0.01 | 0.038 | |
| Na2WO4 x 2 H2O | 10.00 | mg | 0.01 | 0.03 | |
| Fe(NH4)2(SO4)2 x 6 H2O | 10.00 | mg | 0.01 | - | |
| FeSO4 x 7 H2O | 20.00 | mg | 0.02 | 0.071 | |
| Trace mineral solution | 10.00 | ml | - | - | |
| Trace vitamins | 1.00 | ml | - | - | |
| NaHCO3 | 2.00 | g | 1.978 | 23.549 | |
|
Sulfur
(powder) |
30.00 | g | 29.674 | 925.276 | |
| Na2S x 9 H2O | 0.50 | g | 0.495 | 2.059 | |
| Resazurin | 1.00 | mg | 9.9e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix ingredients, except Trace vitamins, NaHCO3, sulfur and Na2S x 9H2O, adjust pH to 6.0, and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution, and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Separately autoclave 5% Na2S x 9H2O solution under a N2 atmosphere. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.6 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Aseptically and anaerobically add the sterile Na2S x 9H2O solution. Pressurize the inoculated bottle to 300 kPa H2-CO2 (4:1, v/v). | |||||
Solution occurs in 2
other media (first defined in Medium J369).
| 1 Use Trace mineral solution of Medium No. 324 supplemented with 0.01 g/L SrCl2 x H2O, 0.01 g/L NaBr and 0.01 g/L KI. |
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.0 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Thioreductor micantisoli DSM 16661
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sulfur | 29.674 | 925.276 |
| NaCl | 29.674 | 507.762 |
| MgCl2 x 6 H2O | 4.135 | 20.337 |
| MgSO4 x 7 H2O | 3.363 | 13.644 |
| NaHCO3 | 1.978 | 23.549 |
| Show more | ||
