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JCM 280 . THERMOCOCCUS MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Thermococcus gorgonarius DSM 10395

This strain uses the standard medium without modifications.

Main sol. J280 #

ml
Solution occurs in 1 other media (first defined in Medium J280).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KCl 0.330 g 0.324 4.34
MgCl2 x 6 H2O 2.800 g 2.745 13.503
MgSO4 x 7 H2O 3.400 g 3.333 13.524
NH4Cl 0.250 g 0.245 4.582
NaCl 25.000 g 24.51 419.401
K2HPO4 0.300 g 0.294 1.689
Yeast extract
(BD-Difco) 		3.000 g 2.941 -
Tryptone
(BD-Difco) 		3.000 g 2.941 41.378
FeSO4 x 7 H2O 0.025 g 0.025 0.088
NaBr 10.000 mg 0.01 0.095
Trace minerals 10.000 ml - -
Trace vitamins 10.000 ml - -
Sulfur
(powder) 		10.000 g 9.804 305.704
Na2S x 9 H2O 0.500 g 0.49 2.041
Resazurin 1.000 mg 9.8e-4 0.004
Distilled water 1000.000 ml - -
1 Mix ingredients except sulfur and Na2S x 9H2O, adjust pH to 7.0-7.2 and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution and autoclave under a N2 gas atmosphere. Distribute the medium and sulfur into culture vessels under a N2 stream, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 7.0-7.2, if necessary.
2 Comment:
3 Most of Thermococcus strains will grow in this medium supplemented with 5.0 g/L maltodextrin and omitted sulfur.

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Archaea
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 26
Thermococcus gorgonarius DSM 10395

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 24.52 419.569
Sulfur 9.804 305.704
MgSO4 x 7 H2O 3.363 13.643
Tryptone 2.941 41.378
Yeast extract 2.941 -
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Cultivation metadata from 26 strains BacDive

pH 6 - 8 1 strain
phyla 2 26 strains
oxygen anaerobe 18 strains
temperature hyperthermophilic 24 strains
temperature thermophilic 2 strains