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JCM 242 . JCM MEDIUM No. 242

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Methanobacterium formicicum DSM 1535

This strain uses the standard medium without modifications.

Main sol. J242 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.30 g 0.294 2.161
K2HPO4 0.30 g 0.294 1.689
(NH4)2SO4 0.30 g 0.294 2.226
NaCl 0.60 g 0.588 10.066
MgSO4 x 7 H2O 0.13 g 0.127 0.517
FeSO4 x 7 H2O 2.00 mg 0.002 0.007
CaCl2 x 2 H2O 8.00 mg 0.008 0.053
Yeast extract
(BD-Difco) 		2.00 g 1.961 -
Trypticase peptone
(BD-BBL) 		2.00 g 1.961 -
Sodium acetate 2.50 g 2.451 29.879
Sodium formate 2.50 g 2.451 36.04
Trace minerals 10.00 ml - -
Trace vitamins 10.00 ml - -
NaHCO3 5.00 g 4.902 58.352
Na2S x 9 H2O 0.50 g 0.49 2.041
L-Cysteine HCl x H2O 0.50 g 0.49 -
Resazurin 1.00 mg 9.8e-4 0.004
Distilled water 1000.00 ml - -
1 Mix ingredients, except NaHCO3, L-Cysteine x HCl x H2O and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of H2-CO2 (80:20), and add NaHCO3 to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20) and seal with butyl rubber stoppers. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions. Pressurize the inoculated vessels to 200 kPa H2-CO2 (80:20).

Trace minerals #

1000
Solution occurs in 97 other media (first defined in Medium J151).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x n H2O 0.50 g 0.5 -
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.10 g 0.1 0.645
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 0.01 g 0.01 0.039
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
Distilled water 1000.00 ml - -
1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 5
Methanobacterium formicicum DSM 1535

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.902 58.352
Sodium formate 2.451 36.04
Sodium acetate 2.451 29.879
Trypticase peptone 1.961 -
Yeast extract 1.961 -
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Cultivation metadata from 5 strains BacDive

phyla 1 5 strains
oxygen anaerobe 4 strains
temperature mesophilic 4 strains
temperature thermophilic 1 strain