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JCM 230 . METHANOSARCINA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Methanosarcina barkeri DSM 800

This strain uses the standard medium without modifications.

Main sol. J230 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.227 g 0.227 1.665
K2HPO4 0.348 g 0.347 1.994
NH4Cl 0.500 g 0.499 9.329
MgSO4 x 7 H2O 0.500 g 0.499 2.025
CaCl2 x 2 H2O 0.250 g 0.25 1.697
NaCl 2.250 g 2.246 38.424
FeSO4 x 7 H2O 2.000 mg 0.002 0.007
Yeast extract
(BD-Difco) 		2.000 g 1.996 -
Casitone
(BD-Difco) 		2.000 g 1.996 -
Trace vitamins 10.000 ml - -
FeCl2 solution 1.000 ml - -
Trace element solution 1.000 ml - -
NaHCO3 0.850 g 0.848 10.098
Methanol 10.000 ml - -
L-Cysteine HCl x H2O 0.300 g 0.299 -
Na2S x 9 H2O 0.300 g 0.299 1.247
Resazurin 1.000 mg 1.0e-3 0.004
Distilled water 980.000 ml - -
1 Mix ingredients, except NaHCO3, methanol, cysteine x HCl and Na2S x 9H2O, and bring to a boil for 5-10 sec. Cool down under a stream of N2-CO2 (80:20), and add NaHCO3 and methanol to the medium. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of N2-CO2 (80:20) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the cysteine x HCl and Na2S x 9H2O solutions.

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

FeCl2 solution #

1000
Solution occurs in 87 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(7.7 M, 25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
Distilled water 990.0 ml - -

Trace element solution #

1000
Solution occurs in 89 other media (first defined in Medium J187).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnCl2 70 mg 0.07 0.514
MnCl2 x 4 H2O 100 mg 0.1 0.505
H3BO3 6 mg 0.006 0.097
CoCl2 x 6 H2O 190 mg 0.19 1.463
CuCl2 x 2 H2O 2 mg 0.002 0.012
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 36 mg 0.036 0.149
Distilled water 1000 ml - -

Metadata

Last modified: 29.10.21
Source: JCM
Taxonomic range:  Archaea
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Anaerobic workbench, Autoclave
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Associated strains

All strains for this medium: 4
Methanosarcina barkeri DSM 800

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 2.246 38.424
Casitone 1.996 -
Yeast extract 1.996 -
NaHCO3 0.848 10.098
NH4Cl 0.499 9.329
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature mesophilic 4 strains