J14
14 .
EG MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Bifidobacterium JCM 7027
This strain uses the standard medium without modifications.
Main sol. J14 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
2.4 | g | 2.4 | - | |
|
Proteose peptone no. 3
(BD-Difco) |
10.0 | g | 10 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 5 | - | |
| Na2HPO4 | 4.0 | g | 4 | 28.177 | |
| Glucose | 1.5 | g | 1.5 | 8.326 | |
|
Starch
(soluble) |
0.5 | g | 0.5 | - | |
| L-Cystine | 0.2 | g | 0.2 | 0.832 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| Horse blood | 50.0 | ml | - | - | |
| Distilled water | 950.0 | ml | - | - | |
| 1 Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.7 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 942 |
Bifidobacterium JCM 7027
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Proteose peptone no. 3 | 10 | - |
| Yeast extract | 5 | - |
| Na2HPO4 | 4 | 28.177 |
| Lab-Lemco beef extract | 2.4 | - |
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Cultivation metadata from 942 strains
| pH | 6 - 8 | 37 strains |
| pH | 4 - 6 | 15 strains |
| pH | 8 - 10 | 10 strains |
| pH | 10 - 12 | 1 strain |
| phyla | 8 | 927 strains |
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