J13
13 .
BL AGAR (GLUCOSE BLOOD LIVER AGAR)
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Bifidobacterium JCM 7027
This strain uses the standard medium without modifications.
Main sol. J13 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
3.0 | g | 2.857 | - | |
|
Proteose peptone no. 3
(BD-Difco) |
10.0 | g | 9.524 | - | |
|
Trypticase peptone
(BD-BBL) |
5.0 | g | 4.762 | - | |
|
Phytone peptone
(BD-BBL) |
3.0 | g | 2.857 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 4.762 | - | |
|
Liver extract
(see below) |
150.0 | ml | - | - | |
| Glucose | 10.0 | g | 9.524 | 52.863 | |
|
Starch
(soluble) |
0.5 | g | 0.476 | - | |
| Solution A | 10.0 | ml | - | - | |
| Solution B | 5.0 | ml | - | - | |
| Tween 80 | 1.0 | g | 0.952 | 0.728 | |
|
Agar
(BD-Difco, Bacto ) |
15.0 | g | 14.286 | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
| Horse blood | 50.0 | ml | - | - | |
| Distilled water | 825.0 | ml | - | - | |
| 1 Adjust pH to 7.2. | |||||
| 2 To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| K2HPO4 | 10 | g | 100 | 574.132 |
| KH2PO4 | 10 | g | 100 | 734.829 |
| Distilled water | 100 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| MgSO4 x 7 H2O | 4.0 | g | 40 | 162.288 | |
| NaCl | 0.2 | g | 2 | 34.223 | |
| FeSO4 x 7 H2O | 0.2 | g | 2 | 7.194 | |
| MnSO4 x n H2O | 0.2 | g | 2 | - | |
| Distilled water | 100.0 | ml | - | - | |
| 1 Mix ingredients except horse blood and adjust pH to 7.2. After autoclaving, cool to 50C, and aseptically add 50.0 ml of horse blood. | |||||
| 2 Comment: Commercial BL agar (Nissui) is also available. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 750 |
Bifidobacterium JCM 7027
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 14.286 | - |
| Glucose | 9.524 | 52.863 |
| Proteose peptone no. 3 | 9.524 | - |
| Trypticase peptone | 4.762 | - |
| Yeast extract | 4.762 | - |
| Show more | ||
Cultivation metadata from 750 strains
| pH | 6 - 8 | 14 strains |
| pH | 4 - 6 | 9 strains |
| pH | 8 - 10 | 2 strains |
| phyla | 5 | 749 strains |
| oxygen | anaerobe | 184 strains |
| Show more | ||