13 .
BL AGAR (GLUCOSE BLOOD LIVER AGAR)
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Selected strain: Bifidobacterium breve DSM 20091
This strain uses the standard medium without modifications.
Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Lab-Lemco beef extract
(Oxoid) |
3.0 | g | ||
|
|
Proteose peptone no. 3
(BD-Difco) |
10.0 | g | ||
|
|
Trypticase peptone
(BD-BBL) |
5.0 | g | ||
|
|
Phytone peptone
(BD-BBL) |
3.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
5.0 | g | ||
|
|
Liver extract
(see below) |
150.0 | ml | ||
|
|
Glucose | 10.0 | g | ||
|
|
Starch
(soluble) |
0.5 | g | ||
|
|
Solution A | 10.0 | ml | ||
|
|
Solution B | 5.0 | ml | ||
|
|
Tween 80 | 1.0 | g | ||
|
|
Agar
(BD-Difco, Bacto ) |
15.0 | g | ||
|
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | ||
|
|
Horse blood | 50.0 | ml | ||
|
|
Distilled water | 825.0 | ml | ||
| 1 Adjust pH to 7.2. | |||||
| 2 To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter. | |||||
Solution B #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 4.0 | g | ||
|
|
NaCl | 0.2 | g | ||
|
|
FeSO4 x 7 H2O | 0.2 | g | ||
|
|
MnSO4 x n H2O | 0.2 | g | ||
|
|
Distilled water | 100.0 | ml | ||
| 1 Mix ingredients except horse blood and adjust pH to 7.2. After autoclaving, cool to 50C, and aseptically add 50.0 ml of horse blood. | |||||
| 2 Comment: Commercial BL agar (Nissui) is also available. | |||||