J1293
1293 .
MMJF MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1293 #
ml
Equipment needed: Autoclave
Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified MJ synthetic seawater C | 1000.0 | ml | - | - | |
| Ferrihydrite | 2.0 | g | 1.976 | - | |
| 1 Mix components and adjust pH to 6.5. Distribute the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (filter-sterilized): | |||||
|
NaHCO3
(8%) |
12.5 | ml | - | - | |
| 2 Check final pH of the medium to be 6.5. | |||||
Solution occurs in 1
other media (first defined in Medium J1293).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaCl | 25.00 | g | 24.753 | 423.554 |
| MgCl2 x 6 H2O | 4.20 | g | 4.158 | 20.454 |
| MgSO4 x 7 H2O | 3.40 | g | 3.366 | 13.658 |
| KCl | 0.50 | g | 0.495 | 6.641 |
| NH4Cl | 0.25 | g | 0.248 | 4.628 |
| K2HPO4 | 0.14 | g | 0.139 | 0.796 |
| CaCl2 x 2 H2O | 0.70 | g | 0.693 | 4.714 |
| FeSO4 x 7 H2O | 0.02 | g | 0.02 | 0.071 |
| Trace mineral solution | 10.00 | ml | - | - |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 1
other media (first defined in Medium J1293).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Trace minerals | 1000.000 | ml | - | - |
| NiCl2 x 6 H2O | 0.075 | g | 0.075 | 0.579 |
| Na2SeO3 x 5 H2O | 0.050 | g | 0.05 | 0.19 |
| Na2WO4 x 2 H2O | 0.100 | g | 0.1 | 0.303 |
Solution occurs in 97
other media (first defined in Medium J151).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.50 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.00 | g | 3 | 12.172 | |
| MnSO4 x n H2O | 0.50 | g | 0.5 | - | |
| NaCl | 1.00 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.10 | g | 0.1 | 0.645 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.01 | g | 0.01 | 0.04 | |
| AlK(SO4)2 | 0.01 | g | 0.01 | 0.039 | |
| H3BO3 | 0.01 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.01 | g | 0.01 | 0.041 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Defined medium |
| Final pH: | 6.5 |
| Equipment needed: | Anaerobic workbench, Autoclave, Filter |
| Edit in Medium builder | |