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JCM 1230 . NSY MEDIUM FOR POLYNUCLEOBACTER

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J1230 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nutrient broth 1.0 g 1 -
Phytone peptone
(BD-BBL) 		1.0 g 1 -
Yeast extract
(BD-Difco) 		1.0 g 1 -
MgSO4 x 7 H2O 75.0 mg 0.075 0.304
Ca(NO3)2 x 4 H2O 43.0 mg 0.043 0.182
NaHCO3 16.0 mg 0.016 0.19
KCl 5.0 mg 0.005 0.067
K2HPO4 3.7 mg 0.004 0.021
Trace element solution SL-4 0.1 ml - -
Distilled water 1000.0 ml - -
Agar 15.0 g 15 44.599
1 Adjust pH to 7.2.

Trace element solution SL-4 #

1000
Solution occurs in 21 other media (first defined in Medium J340).
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 0.5 g 0.5 1.711
FeSO4 x 7 H2O 0.2 g 0.2 0.719
Trace element solution SL-6 100.0 ml - -
Distilled water 900.0 ml - -

SL-6 trace element solution #

1000
Solution occurs in 10 other media (first defined in Medium J167).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 Adjust pH to 3.6.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
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Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Nutrient broth 1 -
Yeast extract 1 -
Phytone peptone 1 -
MgSO4 x 7 H2O 0.075 0.304
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Cultivation metadata from 4 strains BacDive

phyla 1 5 strains
nutrition chemoorganotroph 1 strain
oxygen obligate aerobe 2 strains
oxygen aerobe 1 strain
temperature mesophilic 5 strains
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