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JCM 1196 . ACIDIHALOBACTER MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Acidihalobacter ferrooxydans DSM 14175

This strain uses the standard medium without modifications.

Solution A #

900
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 15.0 g 16.667 285.193
(NH4)2SO4 0.2 g 0.222 1.682
MgSO4 x 7 H2O 0.4 g 0.444 1.803
K2HPO4 0.1 g 0.111 0.638
Distilled water 900.0 ml - -
1 Adjust pH 1.7 with H2SO4 and autoclave.

Solution B #

100
Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 13.9 g 139 499.973
Distilled water 100.0 ml - -
1 Adjust pH to 1.25 and autoclave at 115C for 10 min or filter-sterilize.

Solution C #

10
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2S4O6 0.3 g 30 -
Distilled water 10.0 ml - -
1 Sterilize by filtration.

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 0.1 g 10 -
Distilled water 10.0 ml - -
1 Sterilize by autoclaving.
2 Aseptically combine solutions A and B after cooling. Then, add solutions C and D to the medium.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 1.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 2
Acidihalobacter ferrooxydans DSM 14175

No modifications necessary for this strain.

Cultivation metadata from 2 strains BacDive

halophily halotolerant 2 strains
pH 0 - 2 2 strains
pH 2 - 4 2 strains
phyla 1 2 strains
nutrition chemolithoautotroph 2 strains
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