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JCM 1129 . HESP1 MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Anaerocolumna xylanovorans DSM 12503

This strain uses the standard medium without modifications.

Main sol. J1129 #

ml
Equipment needed: Autoclave Hungate tubes Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 1.0 g 0.921 17.215
K2HPO4 0.3 g 0.276 1.586
KH2PO4 0.3 g 0.276 2.03
MgCl2 x 6 H2O 0.2 g 0.184 0.906
CaCl2 x 2 H2O 0.1 g 0.092 0.626
KCl 0.1 g 0.092 1.235
NaCl 0.6 g 0.552 9.454
Yeast extract 1.0 g 0.921 -
Trace element solution SL-10 1.5 ml - -
Resazurin 0.5 mg 4.6e-4 0.002
L-Cysteine HCl x H2O 0.5 g 0.46 -
Distilled water 1000.0 ml - -
1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized). Prior to use adjust pH to 7.0 - 7.2.
NaHCO3
(*, 8%) 		56.0 ml - -
Fructose
(1 M) 		20.0 ml - -
Na2S x 9 H2O
(5%) 		8.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 20 other media (first defined in Medium J433).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, 7.7 M) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Anaerobic workbench, Autoclave, Filter, Hungate tubes
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Associated strains

All strains for this medium: 1
Anaerocolumna xylanovorans DSM 12503

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NH4Cl 0.921 17.215
Yeast extract 0.921 -
NaCl 0.552 9.454
L-Cysteine HCl x H2O 0.46 -
KH2PO4 0.276 2.03
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain