J1029
1029 .
RUMEN BACTERIA MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Butyrivibrio fibrisolvens DSM 3071
This strain uses the standard medium without modifications.
Main sol. J1029 #
ml
Equipment needed: Anaerobic workbench
Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mineral solution | 38.00 | ml | - | - | |
| K2HPO4 | 0.30 | g | 0.3 | 1.721 | |
|
Trypticase peptone
(BD-BBL) |
2.00 | g | 1.998 | - | |
| Yeast extract | 0.50 | g | 0.5 | - | |
| VFA solution | 3.10 | ml | - | - | |
| Hemin | 1.00 | mg | 1.0e-3 | 0.002 | |
| Glucose | 0.50 | g | 0.5 | 2.773 | |
| Maltose | 0.50 | g | 0.5 | 1.459 | |
| Cellobiose | 0.50 | g | 0.5 | 1.459 | |
| Starch , soluble | 0.50 | g | 0.5 | - | |
| Glycerol | 0.50 | g | 0.5 | 5.424 | |
| Na2CO3 | 4.00 | g | 3.996 | 37.702 | |
| Resazurin | 1.00 | mg | 1.0e-3 | 0.004 | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.25 | - | |
| Na2S x 9 H2O | 0.25 | g | 0.25 | 1.04 | |
| Distilled water | 960.00 | ml | - | - | |
| 1 Adjust pH to 6.7 - 6.8; gas atmosphere: 100% CO2. | |||||
| 2 Dissolve ingredients except carbohydrates, Na2CO3, cysteine and sodium sulfide in distilled water, boil for 5 min, then cool to room temperature while gassing with 100% CO2. Add Na2CO3 and equilibrate the medium with the gas to pH 6.8. Distribute under CO2 in rubber-stoppered tubes (i.e. Hungate tubes) and autoclave. Thereafter add carbohydrate mixture, cysteine and sodium sulfide from concentrated sterile stock solutions (prepared anaerobically under N2 gas and autoclaved). | |||||
Solution occurs in 4
other media (first defined in Medium 330).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| KH2PO4 | 6.0 | g | 6 | 44.09 |
| NaCl | 12.0 | g | 12 | 205.339 |
| (NH4)2SO4 | 6.0 | g | 6 | 45.406 |
| CaCl2 x 2 H2O | 1.6 | g | 1.6 | 10.883 |
| MgSO4 x 7 H2O | 2.5 | g | 2.5 | 10.143 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium J133).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Acetic acid | 17 | ml | - | - |
| Propionic acid | 6 | ml | - | - |
| n-Butyric acid | 4 | ml | - | - |
| iso-Butyric acid | 1 | ml | - | - |
| n-Valeric acid | 1 | ml | - | - |
| iso-Valeric acid | 1 | ml | - | - |
| DL-alpha-Methyl butyric acid | 1 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.8 |
| Equipment needed: | Anaerobic workbench, Autoclave, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Butyrivibrio fibrisolvens DSM 3071
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2CO3 | 3.996 | 37.702 |
| Trypticase peptone | 1.998 | - |
| Glycerol | 0.5 | 5.424 |
| Glucose | 0.5 | 2.773 |
| Maltose | 0.5 | 1.459 |
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