C59
59 .
MWC
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Mail sol. C59 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
TES
(dry buffer) |
0.115 | g | 0.115 | 0.502 | |
|
CaCl2 x 2 H2O
(3.68 g/100 ml stock solution) |
1.000 | ml | - | - | |
|
MgSO4 x 7 H2O
(3.7 g/100 ml stock solution) |
1.000 | ml | - | - | |
|
NaHCO3
(1.26 g/100 ml stock solution) |
1.000 | ml | - | - | |
|
K2HPO4 x 3 H2O
(1.14 g/100 ml stock solution) |
1.000 | ml | - | - | |
|
NaNO3
(8.5 g/100 ml stock solution) |
1.000 | ml | - | - | |
|
Na2SiO3 x 5 H2O
(2.12 g/100 ml stock solution) |
1.000 | ml | - | - | |
| Combined trace element solution* | 0.100 | ml | - | - | |
| MWC Vitamin mix** | 1.000 | ml | - | - | |
| Deionized water | 1000.000 | ml | - | - | |
| 1 Make up to 1 litre with deionized water. Autoclave at 15 psi for 15 minutes. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 4.360 | g | 43.6 | 129.682 |
| FeCl3 x 6 H2O | 3.150 | g | 31.5 | 116.539 |
| CuSO4 x 5 H2O | 0.010 | g | 0.1 | 0.401 |
| ZnSO4 x 7 H2O | 0.022 | g | 0.22 | 0.765 |
| CoCl2 x 6 H2O | 0.010 | g | 0.1 | 0.77 |
| MnCl2 x 4 H2O | 0.180 | g | 1.8 | 9.095 |
| Na2MoO4 x 2 H2O | 0.006 | g | 0.06 | 0.248 |
| H3BO3 | 1.000 | g | 10 | 161.708 |
Solution occurs in 4
other media (first defined in Medium C34).
| 1 First make primary stocks of Cyanocobalamin and Biotin. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Cyanocobalamine
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
|
Biotin
(0.01 g/100 ml stock solution) |
1.00 | ml | - | - | |
| 2 Dispense any excess primary stocks into 1 ml aliquots and freeze. | |||||
| Thiamine HCl | 0.02 | g | 0.1 | 0.296 | |
Metadata
| Last modified: | 07.10.22 |
| Source: | CCAP |
| Synonyms: | Modified Woods Hole Medium |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Reference: | doi.org/10.1111/j.1529-8817.1972.tb03995.x |
| Edit in Medium builder | |