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CCAP 20 . C Medium, Modified

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C20 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 0.10 g 0.1 0.989
Ca(NO3)2 x 4 H2O 0.15 g 0.15 0.635
Sodium glycerophosphate 0.05 g 0.05 -
MgSO4 x 7 H2O 0.04 g 0.04 0.162
Tris(hydroxymethyl)aminomethane 0.50 g 0.5 4.127
Trace element solution 3.00 ml - -
Vitamin B1 solution 1.00 ml - -
Vitamin B12 solution 1.00 ml - -
Biotin solution 10.00 ml - -
Distilled water 1000.00 ml - -
Agar , for solid medium 15.00 g 15 -
1 Dissolve the TRIS buffer into 900 ml distilled water, then add the remaining components. Bring to 1 litre with distilled water. For agar add 15 g per litre Bacterial Agar. Autoclave at 15 psi for 15 minutes. Final pH should be 7.5.

Trace element solution #

1000
Solution occurs in 2 other media (first defined in Medium C20).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.00 ml - -
Na2-EDTA 0.75 g 0.75 2.231
1 Add to 1000 ml of distilled water 0.75 g Na2EDTA and the minerals in exactly the following sequence:
FeCl3 x 6 H2O 97.00 mg 0.097 0.359
MnCl2 x 4 H2O 41.00 mg 0.041 0.207
ZnCl2 x 6 H20 5.00 mg 0.005 0.02
CoCl2 x 6 H2O 2.00 mg 0.002 0.015
Na2MoO4 x 2 H2O 4.00 mg 0.004 0.017

Vitamin B1 solution #

100
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine hydrochloride
(Vitamin B1) 		0.12 g 1.2 3.558
1 Filter sterile

Vitamin B12 solution #

100
Solution occurs in 11 other media (first defined in Medium C1).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Cyanocobalamine
(Vitamin B12) 		0.1 g 1 0.738
1 Take 1 ml of this solution and add 99 ml Deionised water. Filter sterile.

Biotin solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.005 g 0.05 0.205
Distilled water 100.000 ml - -
1 Filter sterile.

Metadata

Last modified: 07.10.22
Source: CCAP
Medium type: Defined medium
Final pH: 7.5
Description:

Reference: Ichimura T (1971) Sexual cell division and conjugation­papilla formation in sexual reproduction of Closterium strigosum. In: Nishizawa K, Arasaki S, Chihara M, Hirose H, Nakamura V, Tsuchiya Y eds. Proceedings of the Seventh International Seaweed Symposium, Sapporo, Japan, August 8­12, 1971. Proceedings of the Seventh International Seaweed Symposium. University of Tokyo Press, Tokyo, pp. 208­14. - Watanabe MM, Kawachi M, Hiroki M & Kasai F (2000) NIES Collection List of Strains. Sixth Edition, 2000, Microalgae and Protozoa. Microbial Culture Collections, National Institute for Environmental Studies, Tsukuba, Japan, 159 pp.
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Associated strains

All strains for this medium: No strains found

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 -
Tris(hydroxymethyl)aminomethane 0.5 4.127
Ca(NO3)2 x 4 H2O 0.15 0.635
KNO3 0.1 0.989
Sodium glycerophosphate 0.05 -
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