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CCAP 18 . BG11₀

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Mail sol. C18 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4
(2 g/500 ml stock solution) 		10 ml - -
MgSO4 x 7 H2O
(3.75 g/500 ml stock solution) 		10 ml - -
CaCl2 x 2 H2O
(1.8 g/500 ml stock solution) 		10 ml - -
Citric acid
(0.3 g/500 ml stock solution) 		10 ml - -
Ammonium ferric citrate
(green, 0.3 g/500 ml stock solution) 		10 ml - -
Na2-EDTA
(0.05 g/500 ml stock solution) 		10 ml - -
Na2CO3
(1 g/500 ml stock solution) 		10 ml 25.3 238.706
Trace metal solution 1 ml - -
Deionized water 1000 ml - -
1 This medium is standard BG11 but omitting NaNO3.
2 Make up to 1 litre with deionized water. Adjust pH to 7.1 with 1M NaOH or HCl. For agar add 15.0 g per litre of Bacteriological Agar (Thermo Scientific™ Oxoid™ Agar No.1). Autoclave at 15 psi for 15 minutes.
3 Due to precipitation, larger volumes require stocks of citric acid and ammonium ferric citrate to be autoclaved separately in 100ml deionized water or alternatively they can be autoclaved separately in test tubes and added to sterile medium in the airflow cabinet.

Trace metal solution #

1000
Solution occurs in 1 other media (first defined in Medium C17).
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 2.86 g 2.86 46.248
MnCl2 x 4 H2O 1.81 g 1.81 9.146
ZnSO4 x 7 H2O 0.22 g 0.22 0.765
Na2MoO4 x 2 H2O 0.39 g 0.39 1.612
CuSO4 x 5 H2O 0.08 g 0.08 0.32
Co(NO3)2 x 6 H2O 0.05 g 0.05 0.172

Metadata

Last modified: 07.10.22
Source: CCAP
Synonyms: BG110, Blue-Green Medium modified for nitrogen fixing cyanobacteria, BG110, BG110, BG11₀
Medium type: Defined medium
Final pH: 7.1
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Associated strains

All strains for this medium: 40

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2CO3 25.3 238.706
H3BO3 0.003 0.046
MnCl2 x 4 H2O 0.002 0.009
Na2MoO4 x 2 H2O 3.9e-4 0.002
ZnSO4 x 7 H2O 2.2e-4 7.7e-4
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