This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
D-Glucose OR Sorbitol | 3.6 | g | 3.6 | - | |
NH4Cl | 0.6 | g | 0.6 | 11.217 | |
KH2PO4 | 1.0 | g | 1 | 7.348 | |
MgSO4 x 7 H2O | 0.4 | g | 0.4 | 1.623 | |
NaCl | 0.4 | g | 0.4 | 6.845 | |
CaCl2 x 2 H2O | 0.5 | mg | 5.0e-4 | 0.003 | |
Trace element solution SL-4 | 1.0 | ml | - | - | |
Vitamin solution
(10 x conc.) |
5.0 | ml | - | - | |
Distilled water | 1000.0 | ml | - | - | |
1 pH 6.8. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Vitamin B12 | 50 | mg | 0.05 | 0.037 | |
Pantothenic acid | 50 | mg | 0.05 | 0.228 | |
Riboflavin | 50 | mg | 0.05 | 0.133 | |
Pyridoxamine hydrochloride | 10 | mg | 0.01 | 0.049 | |
Biotin | 20 | mg | 0.02 | 0.082 | |
Folic acid | 20 | mg | 0.02 | 0.045 | |
Nicotinic acid | 25 | mg | 0.025 | 0.203 | |
Nicotine amide | 25 | mg | 0.025 | 0.205 | |
alpha-lipoic acid | 50 | mg | 0.05 | 0.242 | |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 | |
Thiamine-HCl x 2 H2O | 50 | mg | 0.05 | 0.134 | |
Distilled water | 1000 | ml | - | - | |
1 Stir for some hours, filter sterilize the solution. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na2-EDTA | 0.50 | g | 0.5 | 1.487 | |
FeSO4 x 7 H2O | 0.20 | g | 0.2 | 0.719 | |
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 | |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 | |
H3BO3 | 0.30 | g | 0.3 | 4.851 | |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 | |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 | |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 | |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 6.8 |
Equipment needed: | Filter |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
3.6 | - | |
KH2PO4 | 1 | 7.348 |
NH4Cl | 0.6 | 11.217 |
NaCl | 0.4 | 6.845 |
MgSO4 x 7 H2O | 0.4 | 1.623 |
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